Optimal an extracellular alkaline protease production condition for Pseudomonas aeruginosa

Production of alkaline protease by Pseudomonas aeruginosa.

A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.

Production of Extracellular Protease and Determination of Optimal Condition by Bacillus Licheniformis BBRC 100053 (RESEARCH NOTE)

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...

In vivo bacterial protease production during Pseudomonas aeruginosa corneal infection.

PURPOSE To establish if active pseudomonal proteases are present in vivo during corneal infection with Pseudomonas aeruginosa and to determine if the mouse strains used in these and previous studies have the ability to mount a nonocular antibody response to the purified proteases because antibodies to the bacterial proteases were not detected previously during in vivo ocular infection. METHOD...

Inhibition of Pseudomonas aeruginosa slime production by staphylococcal extracellular product.

Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited gram-negative bacteria in addition to inhibiting a variety of gram-positive bac...

Screening of Thraustochytrids for alkaline protease production